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Potential therapeutic interventions, such as folinic acid and antioxidant therapy, have been identified as promising strategies to counteract MTX-induced hepatotoxicity (Created by BioRender.com)

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Especially, the transcriptional downregulation of ODC1 by siRNA was shown to lead to hypomethylation of LINE-1 retrotransposons, to induce their transcriptional activity and DNA double-strand breaks in short-term cultivated primary bladder cells [70]

Moreover, culture growth was slowed at 150 g/ml 5-FOA (Figure S2C)