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A direct proteomic method to detect proteins with disulfide bonds and called Redox 2D-PAGE or diagonal SDS-PAGE is based on the differential migration of proteins containing intra-or intermolecular disulfides under non-reducing (first dimension) and reducing (second dimension) conditions ( Most of the current methods designed to detect reactive cysteines and redox PTMs are indirect and are based on the differential alkylation of reduced and oxidized thiols ( Figure 2 )

This work was funded by Lingbi County Edible Mushroom Industry Development Science and Technology Mission

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[23] In more severe or prolonged cases of pernicious anemia, nerve cell damage may occur

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