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Animal studies have revealed that mild to moderate acoustic overexposure, even when causing only a temporary threshold shift (TTS), can immediately and permanently induce cochlear synaptopathycharacterized by the loss of synaptic connections between cochlear hair cells and auditory nerve fibers, without any loss of the hair cells themselves 16,17,18

For some samples of lees (1 to 4 and 7), GSH was present in higher concentrations than GSSG, while the opposite was observed for samples 5-6 and 8 to 15

A solvent blank, which consisted of a water: methanol (90:10 v/v) solution, and a process blank were analysed at the beginning and end of each batch

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