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[DOI] [PubMed] [Google Scholar] 271.Tonyushkina K and Nichols JH, J

doi: 10.4158/EP13180.RA 16 LeungAMAvramAMBrennerAVDuntasLHEhrenkranzJHennesseyJVet al

Following induction, bacterial cells were pelleted by centrifugation at 5000 g for 20 min at 4 C, suspended in buffer A (20 mM Tris-HCl pH 8.0, 1 M NaCl, 10% glycerol, 0.1% -mercaptoethanol, 0.2% Triton X-100, 10 mM imidazole and 1 mM PMSF) and disrupted by sonication

They take it at night because it's convenient, or with breakfast because they forget to dose earlier, and when results are inconsistent they assume the peptide doesn't work or their batch is underdosed

Early intervention (within days to weeks) potentially preventing progression to osteoarthritis