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G.VellaR

Part of this effect is mediated by the upregulation of miR-125b, which amplifies copper-dependent pro-inflammatory signaling [209]

Real-time PCR was performed in duplicate for each sample using the ABI PRISM 7500 Real-Time PCR System and SYBR Green Master Mix (Applied Biosystems) according to the manufacturers instructions

The accumulation of mAPP and A has been shown to increase mitochondrial fission and reduce fusion in hippocampal neurons, characterized by elevated levels of fission proteins Drp1 and Fis1, alongside decreased expression of fusion proteins Mitofusin 1, Mitofusin 2, and Optic Atrophy 1 (Reddy et al