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doi: 10.7759/cureus.55352 43

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After 48 h, the medium was removed, 400 l of ice-cold 80% methanol was added and the cells were incubated at 80 C for 15 min

Individuals most likely to benefit include: Those with pernicious anemia People with gastrointestinal disorders (e.g., Crohns disease, celiac disease, chronic gastritis) Patients following bariatric (weight loss) surgery Older adults with absorption difficulties Strict vegetarians or vegans at risk for deficiency People taking medications that can decrease vitamin B12 levels Laboratory testing usually confirms B12 deficiency before starting injections

CascalesASanchez-VegaBNavarroNPastor-QuiranteFCorralJVicenteVet alClinical and genetic determinants of anthracycline-induced cardiac iron accumulation