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Kangen machines work through electrolysis passing an electrical current through water to separate it into alkaline and acidic streams

N Engl J Med 2020;382:150719

MTA1 -knockout or MTA1 -overexpressed cells were seeded into 384-well assay plates using a Multidrop TM Combi reagent dispenser (Thermo Fisher Scientific) at a density of 1,000 cells per well in 50 l of RPMI medium supplemented with 10% FBS, 2% L-glutamine, 1% penicillinstreptomycin and 0.1% amphotericin B

Verhaart IEC, Robertson A, Wilson IJ, Aartsma-Rus A, Cameron S, Jones CC, Cook SF, Lochmller H (2017) Prevalence, incidence and carrier frequency of 5q-linked spinal muscular atrophyA literature review