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Metabolic activity was determined using a WST1 assay (Cayman) according to the manufactures protocol, after exposure to increasing concentrations of cuprizone (0250 M) for up to 72 h, or 20 M peroxide as positive control

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These are massive doses compared to the amounts found in the average multivitamin tablets (which are set according to the recommended daily intakes for adult males in the UK at around 1.4mg for B6 and 1.5g for B12)

In support of this increased NK cytotoxic potential, the expression of the NK cytotoxic effector-associated gene perforin, as determined by flow-cytometric analysis or quantitative real-time PCR analysis in CD56 + NK cells, was also significantly increased in the above IGF-1-induced CD34 + -derived NK cells compared with the cells without IGF-1 (Fig

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