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The Nature Scientific Reports 2016 study confirmed that GHK-Cu produced no cytotoxicity to human keratinocytes at concentrations up to 5,800 micromolar over 72 hours and did not induce inflammatory biomarkers

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Rather, mice with the highest plasma concentrations of TMAO had the most plaque at the aortic root, whereas those with suppressed gut microbes and lacking TMA/TMAO showed no increase in atherosclerosis