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Gently swirl never shake or vortex as this denatures the 191aa protein

[DOI] [PMC free article] [PubMed] [Google Scholar] 104.Dixon S.J., Lemberg K.M., Lamprecht M.R., Skouta R., Zaitsev E.M., Gleason C.E., Patel D.N., Bauer A.J., Cantley A.M., Yang W.S., et al

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J Biochem Toxic 19:119128 Al-Qenaei A, Yiakouvaki A, Reelfs O, Santambrogio P, Levi S, Hall ND, Tyrrell RM, Pourzand C (2014) Role of intracellular labile iron, ferritin, and antioxidant defence in resistance of chronically adapted Jurkat T cells to hydrogen peroxide

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