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doi: 10.3390/molecules30030652 236 CaputoMBonaELeoneISamMNuzzoAFerreroAet al

Qualified samples were subjected to overnight hybridization reaction at 65C, where 5 l of total RNA samples were combined with 3 l of nCounter reporter CodeSet in 5 l of hybridization buffer and 2 l of nCounter capture ProbeSet for a total reaction volume of 15 l and incubated for 20 h

Some patients experience an immediate boost, while others notice improvements within 24 to 48 hours

The neutralization efficiency of the preservative system in the test sample was determined for the neutralizer: Eugon LT 100 at a sample dilution of 10 1