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In brief, primary human hepatocytes (5 10 3 cells/well) were seeded into 96-well plate and cultured for 24 h

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A 10-point calibration curve was prepared by diluting these stock solutions in methanol, with final concentrations (mol/l): BA, 17.0

In summary, being safe in patients with the inflammatory bowel disease, and devoid of toxic effects (LD1 could be not achieved, limit test negative) (21), gastric pentadecapeptide BPC 157 consistently counteracts analgesia in the mice placed on a hot plate and treated with morphine or morphine + haloperidol

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