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The process of acetylation shields the thiol group from oxidation prior to absorption, ensuring that once inside the cell, S-Acetyl-L-glutathione is hydrolyzed by cytoplasmic thioesterases, thereby replenishing the intracellular GSH pool

The species-specific constants can be obtained as the product of total species concentration and the relative abundance of the respective microspecies

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however, T594 did not form a hydrogen bond with the N atom of the C597 peptide bond (4.1 ) (Figs 4D, S6A-S6B), consistent with the absence of electron density for the final two amino acids, suggesting subunit asymmetry arising from the hydrogen bond interaction of T594