glutathione beads elution What is Agarose? – GoldBio Also available in 96 Purification of proteins fused to

doi:10.1152/japplphysiol.00974.2003 Jentjens R.L.P.G., Venables M.C., and Jeukendrup A.E

Gonzlez-Correa JA, Lpez-Villodres JA, Asensi R, Espartero JL, Rodrguez-Gutirez G, De La Cruz JP

Toxicology and Applied Pharmacology , 11:88103

One round of negative screening was performed by sorting the least fluorescent cells (2.8 10 5 ) in the presence of 1 mM each of tyrosine, l-DOPA, dopamine and 3,4-DHPAA, in order to remove the VanR variants responsive to the substrate and intermediates in hydroxytyrosol biosynthesis

In the in vivo experiments, PVA (with or without drugs) and TSPBA solutions were first kept at room temperature for 0.5 h