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Total, dietary, and supplemental magnesium intakes and risk of all-cause, cardiovascular, and cancer mortality: a systematic review and doseresponse meta-analysis of prospective cohort studies

Neither the NAD+ cohort nor the placebo (saline) cohort experienced any negative side effects throughout the 6 hour infusion (10) At 8 hours following the start of the NAD+ infusion, it was shown that the NAD+ group had significant declines in the liver function enzymes gamma glutamyl transferase, lactate dehydrogenase, and aspartate aminotransferase as well as a large increase in plasma bilirubin (10)

Niu H, Zhang L, Wang B, Zhang GC, Liu J, Wu ZF, et al

Typical protocols involve two to six weeks of use followed by one to four weeks off

They apply whether you are using a pre-filled pen or drawing from a reconstituted vial with a syringe