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Description

It is slightly soluble in acetonitrile, methanol, and water, and its solubility increases when heated

A starter cultured was prepared by inoculating 10 ml of Terrific Broth (Fisher BioReagents) supplemented with 50 g ml 1 kanamycin sulfate (Fisher BioReagents) in a bacterial tube (Corning Falcon) and incubated in an Innova-44 Incubator-Shaker (New Brunswick) at 220 rpm overnight at 30 C (for FGF2 or TGF3) or 37 C (for NRG1)