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[7] Remove medium from this compound-treated neuronal cultures and rinse with D-PBS

A successful pH-sensitive mutant would exhibit a K_D comparable to the wild-type antibody at neutral pH to ensure cell surface binding, but a significantly weakened K_D (markedly increased k_off rate) at acidic pH to facilitate rapid dissociation in the lysosome and enable efficient receptor recycling [76]

Wasmuth, H

doi: 10.1007/s00018-013-1521-z 193 BacciMLoritoNIppolitoLRamazzottiMLutiSRomagnoliSet al

IMMUNE SUPPORT Glutathione is known to strengthen the immune system, especially in people exposed to stress, pollution or UV rays