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After 30 min of blocking with blocking buffer (Dulbeccos PBS containing 0.2% bovine serum albumin, pH 7.4), at RT, the plates were washed 3x with wash buffer (Dulbeccos PBS containing 0.05% Tween 20, pH 7.4)

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Antioxidant defense systems can be broadly categorized into four types: preventive antioxidants, radical scavenging antioxidants, repair antioxidant systems, and cellular signalling antioxidant defense systems

After separating from the ventral ramus, the nerve travels medially before joining a branch from the ramus gray to contribute sympathetic fibers, then passes through the intervertebral foramen and osteofibrous foramen near the pedicle, reentering the spinal canal

FINO(2) initiates ferroptosis through GPX4 inactivation and iron oxidation