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In vitro assessment of hepatoprotective activity using HepG2 cells Cell culture The National Centre for Cell Science in Pune, India, provided the HepG2 cell line, which was cultured in DMEM supplemented with 10% foetal bovine serum, penicillin (100 U/mL), and streptomycin (100 mg/mL) at 37 C in a humidified environment of 5% CO 2

Reactions were performed in 96-well plates (Corning) at 37 C and monitored turbidimetrically using a Synergy HTX microplate reader (BioTek)

Figure 6 Each bar represents the mean SEM, Effect of silymarin on time spent in the open-arm (upper panel) and closed arm (lower panel) of the elevated plus maze

2012 Feb;33(1):77-82

This event causes the relocation of PGAM5 to the mitochondria [74]