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Standard preparation: Reduced glutathione standard (1 mmol/L) was gradient diluted using ultrapure water (e.g., 50 L of its standard was added to 950 L of ultrapure water, diluted to 50 mol/L and further gradient diluted to make a standard curve, i.e., 50/25/12.5/6.25/3.125 mol/L), and then used for data analysis by the standard curve method after subsequent testing with the samples to be tested

For all flow cytometry assays, fluorescence was analyzed using an LSR Fortessa (BD Biosciences) collecting at least 10,000 events per sample

elegans has been reported to extend lifespan compared to WT ( H3K9 Histone H3 lysine 9 can be acetylated (H3K9ac) and methylated (H3K9me), and both modifications have been connected to aging

Lipodystrophy is a condition related to an abnormal distribution of body fat

and 4) at least, IGF-I is growth-hormone dependent [5]