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For vWF assessment, the cells were fixed with 4% paraformaldehyde, washed three times with PBS and incubated in 10% bovine serum albumin for 30 min to block non-specific binding

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Drugs developed based on this hypothesis, however, have not met with much success in recent times

L-DOPA alone contributed to strong recovery of motion phenotypes during initial phase, however, its effect lagged in aged flies which showed movement defects similar to untreated park 13 mutants (Fig