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doi:10.1155/2020/9210572 Grtner R, Gasnier BC, Dietrich JW, Krebs B, Angstwurm MW

Cell proliferation IMR90 (4 10 4 ) were plated in a 24-well plate and placed onto the stage of the Incucyte Live Content Imaging instrument

The two cysteine residues (positions 7 and 14) form an intramolecular disulfide bond that constrains the molecule into a small loop, mirroring the corresponding region of native human growth hormone (residues 177191) with an N-terminal Tyr added for synthesis and labeling reasons

Cell culture, cell treatment, and stable cell line establishment The human renal proximal tubule epithelial cell line HK-2, ccRCC cell lines ACHN, 769-P, 786-O, Caki-1, OSRC-2, and the human umbilical vein endothelial cell line HUVEC were all procured from the American Type Culture Collection (ATCC)

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