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8 mM of reducing agent DTT (Sigma) was then added to the samples and incubated in a thermomixer at 30C for 30 minutes, followed by the addition of 20 mM iodoacetamide (Sigma) for protein carboxylation with incubation at 30C in the dark for 30 minutes

Rosenberger, C

Several biomarkers, such as GPX4 and p53, have been identified for their regulatory effect on ferroptosis, and some compounds targeting these molecules to induce ferroptosis have already been found [9, 10]

The transfected NUGC-4, MKN-45 and NUGC-3 cell lines were seeded into 96-well culture plates and incubated with CCK-8 reagent for 3 h

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