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189, e15e62

doi: 10.1074/jbc.M111.224071 [DOI] [PMC free article] [PubMed] [Google Scholar] 337

Cold diethyl ether (Et 2 O, 5 mL) was added to the filtered resin followed by centrifugation for 10 min in order to precipitate and collect the crude peptide

The maleimide released via retro-Michael addition can rapidly covalently bind with plasma albumin, free cysteine, or glutathione, manifested as maleimide exchange (Figure 11a)

In male Wistar rats experiencing transient ischemic stroke, UA suppresses the upregulation of cerebrocortical Vegfa expression in the acute phase of stroke and reduces the MMP-9/TIMP-1 ratio in the brain cortex, indicating that UA treatment may aid in the downregulation of the VEGF-A/MMP-9 pathway to improve BBB integrity [54]