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Then, cells were incubated with 5 M of MitoSOX TM Red dye in PBS with 0.5% serum at 37 C with 5% CO 2 for 30 min before being assessed in a cytoFLEX cytometer (Beckman Coulter)

10.1007/s11064-011-0690-0 49.Zhu J , Chen Y , Lai J , et al

A useful comparison point is the acylation chemistry described for semaglutide by Lau and colleagues in the Journal of Medicinal Chemistry in 2015 [2], which introduced a C18 diacid linked through a spacer to achieve albumin binding on a GLP-1 scaffold

J Physiol Pharmacol Off J Pol Physiol Soc

Finally, images of the sections were captured under a light microscope ( 400 magnification)