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For nuclei extraction, the cells were incubated with 0.1% (v/v) digitonin (Promega, USA) for 3 min at 4 C and centrifuged for 10 min at 500 x g at 4 C with 1 mL ATAC-RSB, containing 0.1% (v/v) Tween-20

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A 31-gauge needle is thinner than a 27-gauge needle

Future Oncol (2020) 16(18):126987

(C) Fluorescence image of HeLa cells pretreated with N -methylmaleimide (NMM, 1 mM) for 20 min and incubated with probe CPDSA (20 M) for 20 min