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The samples were resuspended in acetonitrile-water (2%:98%) v/v, vortexed for 10 s, and centrifuged (Eppendorf Centrifuge 5425 R) at 14,000 relative centrifugal force (rcf) for 10 min at 4 C

A clinical study demonstrated the effectiveness of melatonin in improving functional and neurological recovery outcomes in patients with acute ischemic stroke when taken within 24 hours of the stroke symptom onset [69]

It was further shown that the suppressed SLC7A11 expression results in decreased cystine uptake and GSH biosynthesis in ARID1A -deficient cancer cells, which induces a vulnerability rendering ARID1A -deficient cancers susceptible for pharmacological inhibition of GSH biosynthesis (Ogiwara et al., 2019)

This leads to the neutralization of their positive electrostatic charge, which may influence protein interactions with substrates, cofactors, and other macromolecules 116,117,118

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