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After homogenization, 1 mL of extraction solution (isopropanol: HO: HCl = 2:1:0.002) was added, and the mixture was sonicated for 30 min at 4 C in the dark using a KQ5200DE ultrasonic cleaner (Kunshan Shumei Ultrasonic Instruments, Suzhou, China)

The potential implications of variation in the remaining genes with borderline marginal significance, including GCLC and ADH5 , are worth mentioning

Most documented cases of toxicity occurred with long-term use and very high doses at 1000mg a day or more

Both systems were protonated at pH 5.5 and 7.0, respectively, and minimized with OPLS- all-atom force fields and solvated with the predefined TIP3P water solvent model

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