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Slides were dried for at least 20 hours at 4C and fluorescent images were obtained with Slidebook 4.2 Digital Microscopy Software driving an Olympus 1X81 inverted confocal microscope with a 60X oil immersion lens, NA 1.4 and resolution 0.280 Dendritic spine density was measured on primary and secondary dendrites at a distance of at least 150 from the soma

Immunofluorescence and immunohistochemistry Testis tissues or TM3 cells were fixed and processed for immunofluorescent or immunohistochemical staining as described previously [35]