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Description

Chest 138 , 929936 (2010)

After mixing with loading buffer, the samples were heated at 100 C for 10 min

Aksu BY

Membranes were blocked for 1 h at room temperature in 1% ECL Advance Blocking Reagent (Cytiva) in PBS with 0.01% Tween20 and then incubated overnight at 4C with primary antibodies diluted in PBS supplemented with 5% BSA, 0.05% sodium azide, and 0.01% Tween20