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Materials and methods Cell culture, regents and plasmids All cell lines (HeLa, MDA-MB-231, and HEK293T) were obtained from the American Type Culture Collection and were maintained in Dulbeccos modified Eagle medium (DMEM, Gibco, 8121298) supplemented with 10% fetal bovine serum (FBS, Gibco, 2176398) and 1% penicillinstreptomycin at 37 C with 5% CO 2

A 2013 study found that high vitamin B levels were linked with a subsequent cancer diagnosis, which was similarly found in a 2020 study that showed that high levels of serum B12 could be linked with the development of solid cancers (tumours)

Experimental evidence for the absorption and tissue distribution of orally administered collagen peptides has been primarily obtained from animal studies

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The DCFH-DA probe, obtained from Solarbio's ROS detection kit (Solarbio, Beijing, China), was diluted 2000-fold for the assay