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Then, the mixture was aliquoted into a Petri culture dish and incubated at 37 C for 20 min allowing for hydrogel formation, followed by culturing for 48 h in the complete culture medium (-MEM supplemented with 10% FBS, 1% antibiotics, 2 mM L-glutamine, 100 mM NEAA, and 550 M 2-ME) in the absence of extra mitogens, e.g., basic fibroblast growth factor (bFGF) and platelet-derived growth factor AA (PDGF-AA), neurotrophic factors, e.g., heregulin--1, and chemicals, e.g., all trans-retinoic acid (RA) and forskolin, all of which are commonly used for the induction of Schwann-like cells from MSCs [33, 34]

Maker HS, Weiss C, Silides DJ, Cohen G

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doi:10.1038/s41574-019-0280-9

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