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Results from the LMER models with the same effect size direction in the discovery and replication cohorts were then meta-analysed using fixed-effects meta-analysis implemented via the R package metafor (v.4.40)

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This pathway is involved in enhancing therapeutic efficacy (e.g., 20(S)-Rg3 combined with sorafenib for hepatocellular carcinoma), reducing toxicity (Astragaloside IV counteracts doxorubicin-induced nephrotoxicity), and reversing drug resistance (Saikosaponin A combined with gemcitabine for intrahepatic cholangiocarcinoma)

Supplementary Table 1

Following successful amplification by monoplex PCR, two distinct pools containing the 21 optimized primer pairs were used for multiplex PCR, maintaining a final primer concentration of 0.1M