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Decreased sensory receptors P2X3and TRPV1 in suburothelial nerve fibers following intradetrusor injections of botulinum toxin for human detrusor overactivity

Proteins were separated with Tris-glycine SDS-PAGE, transferred onto a nitrocellulose membrane, and detected with primary antibodies according to manufacturers instructions, followed by incubation with secondary antibodies conjugated to fluorophores

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