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The lysate was cleared by centrifugation and the protein was purified by Ni-His-affinity chromatography in batch mode

doi: 10.1089/ars.2017.7440 138 WangX.ZhengC.YaoH.GuoY.WangY.HeG.et al

Eur Rev Med Pharmacol Sci 20(11):24682473 ( PMID: 27338076 ) Amor C, Feucht J, Leibold J, Ho YJ, Zhu C, Alonso-Curbelo D, Mansilla-Soto J, Boyer JA, Li X, Giavridis T, Kulick A (2020) Senolytic CAR T cells reverse senescence-associated pathologies