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In another study employing human PCLS, the initial response to LPS exposure was marked by a coordinated release of early release cytokines such as interleukin-1 beta (IL-1), macrophage inflammatory protein-1 beta (MIP-1), and Interferon-gamma (IFN-)

Ytterberg AJ, Zubarev RA, Baumgarten T

Use a steady hand and follow this sequence: Draw the planned volume of bacteriostatic water carefully to limit bubbles in the syringe