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Vessel diameters were measured at the 80 mmHg control pressure in Ca 2+ -free PSS to assess the level of active resting tone in the artery

At the end of the experiment, the cells were washed once with Hanks balanced salt solution (HBSS) and cell viability was assessed by adding 20 L of the CellTiter 96 AQueous One Solution reagent (Promega, Fitchburg, WI) to 100 l HBSS, followed by incubation for 60 min at 37 C, and the absorbance was measured at 490 nm using the Infinite 200 PRO plate-reader (Tekan, Switzerland)

Rosenbloom AL (2009) Mecasermin (recombinant human insulin-like growth factor I)

doi: 10.3390/jcm9123941 251 AzpirozFDubrayCBernalier-DonadilleACardotJMAccarinoASerraJet al

Even, adverse effects are very scarce which are reported above