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After completion of electrophoresis, the samples were transprinted (semi-dry transfer, 15V for 2h) and blocked (blocked with 5% BSA [Bovine serum albumin] in TBST [Tris-Buffered Saline and Tween 20]), and finally the primary and secondary antibodies (SP1 [Cat., No.: sc-420]

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Then 10 mg of protein per sample was separated on 12% SDS-PAGE gels (Sangon Biotech) and subsequently transferred to PVDF membrane (Millipore, Temecula) using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting techniques

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