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Chromatographic peak areas and retention times were analyzed using Multiquant 3.0.2 software, and metabolites were identified by matching retention times with reference standards

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The positioning of the 296 phenol suggests that in the absence of NADPH it orients to block access to the isoalloxazine N5 and prevent non-specific reduction or reoxidation of the flavin, kinetically restricting promiscuous/futile electron transfer out of the TGR population such that even low reduction potential non-native reductants fail to reduce the FAD significantly on timeframes relative to catalysis

doi: 10.1016/j.tox.2020.152665 100 HouJLeiZCuiLHouYYangLAnRet al