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Afterward, cultures were washed with PBS, fixed with ice-cold methanol, and stained with Giemsa staining

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Reprod Biomed Online (2012) 24:496502

Seahorse XP extracellular analysis, Liquid Chromatography/ Mass Spectrophotometry (LC/MS), and 2,7- and Dichlorofluoresin diacetate (DCFDA) assay were used to assess the effects of metformin on cellular bioenergetics, redox status, and redox-related metabolites