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TNF-Exos successfully restored elastin, collagen I, collagen III, IL-6, TNF-, and MMP2 levels in the anterior vaginal wall

Following the incubation, we removed the medium and washed the cells with PBS and with that, all the residuals away

Western blotting Cells and tissues were lysed in RIPA buffer (beyotime, China) containing proteinase inhibitor and phosphatase inhibitor (Thermo Fisher, United States) to extract total proteins and analyzed as previously described [20]

Grondin R, Littrell OM, Zhang Z, Ai Y, Huettl P, Pomerleau F, Quintero JE, Andersen AH, Stenslik MJ, Bradley LH, Lemmon J, ONeill MJ, Gash DM, Gerhardt GA

142 Furthermore, histone methylation and acetylation are closely associated with BCG-induced host-trained immunity against Mtb