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female network iv glutathione thread 24 Frontiers Identification of Chlamydia pneumoniae and

Name: female network iv glutathione thread 24 Frontiers Identification of Chlamydia pneumoniae and
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Cells were grown to mid-log phase (1:20 dilution of overnight cultures into 7 mL fresh LB with 100 g/ml of ampicillin and 10 g/ml gentamicin, grown at 37 C for 1 h) and GST-GalU expression was induced by the addition of 0.1% w/v L-arabinose

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We next performed Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction (ChIPqPCR) targeting conserved PARN promoter regions using IgG or SRF antibodies in MAT2A-overexpressing MNNG/HOS and U-2OS cells, which confirmed that MAT2A enhances PARN transcription by promoting SRF binding to its promoter (Fig