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In comparison to the growth factor protocol, the small molecules appear to be a cheaper and simpler logistical approach for producing HLCs, but an objective comparison demonstrates that a larger number of components are required in the SM protocol ( Methods and materials Small molecules, chemicals, growth factors and antibodies STEMdiff TM Definitive Endoderm Kit (05,111), mTesR (85,850) and ReLeSR (1000,483) were from Stem Cell Technologies (Vancouver, Canada)

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Reconstitution and storage Proper peptide handling maintains potency throughout your protocol