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For histo-morphometric evaluation, the parameters namely seminiferous tubule diameter (STD), thickness of the germinal epithelium of the ST (from the basement membrane to lumen), spermatogonia (Sg), preleptotene, spermatocytes (PLSc), pachytene spermatocytes (PSc), and spermatid cells (Sd) observed in 10 tubules per testicular section and 10 section per groups were measured at 40 magnifications by using calibrated OLYSIA Soft Imaging System GmbH, version 3.2 (Japan).36 Ethical considerations All experimental procedures followed the recommendations provided in the Guide for the Care and Use of Laboratory Animals prepared by the National Academy of Sciences and Published by the National Institute of Health.37 Data presentation and statistical analysis Data were expressed as MeanSEM

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In the freeze-dried material, the basic chemical composition was assessed, the concentration of total iodine and total nitrogen as well as micro- and macroelements were determined