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For labeling experiments with [U- 13 C]-glutamine, the fresh medium contained 4 mM [U- 13 C]-glutamine in glutamine-free DMEM

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New Brunswick (NJ): Johnson & Johnson Consumer Inc

Structural and biochemical analyses of ChaC2 revealed that Glu74 and Glu83 play crucial roles in directing the conformation of the enzyme and in modulating the enzyme activity