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doi: 10.1016/j.actbio.2022.07.055 203 WangLTongLXiongZChenYZhangPGaoYet al

To improve the expression, solubilization, and purification of recombinant proteins, it is therefore advised to alter the culture conditions by switching the medium or the temperature, co-expressing chaperones, adding large hydrophilic fusion tags as well as adding sucrose, raffinose, glycine, betaine and sorbitol during growth for enhancing the recombinant protein expression, solubilization and purification [5, 6]

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The drug is injected into points on the face, back of the neck, back of the neck, and upper arm