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We utilized Superscript III reverse transcriptase enzyme (Invitrogen) with random hexamer primers and 3 g of RNA from each sample to produce cDNA according to the manufacturers protocol

At 1 week after the surgery, transthoracic echocardiography (770 VisualSonics Ultrasound System, 17.5 MHz linear array transducer) was implemented under anesthesia induced by 5% isoflurane and maintained by 2% isoflurane in O 2 with activated charcoal absorption filters (VaporGuard)

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elegans FP::IFDs, also appearing in the axon, nucleus, and in filamentous patterns in the cytoplasm in some neurons (Supplementary Fig

Protective effects of tetramethylpyrazine on myocardial ischemia/reperfusion injury involve NLRP3 inflammasome suppression by autophagy activation