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In biological systems, amino acids and peptides frequently act as ligands because they contain atoms such as nitrogen and oxygen capable of binding metal ions

While the pharmacokinetics of GH fluctuate across a 24-h period with a high peak:trough ratio, this effect is less pronounced in the IGF-I response, where the profile is relatively stable across 24 h, owing to the delay between pharmacokinetics and IGF-I response

All preclinical research must be conducted with oversight from the appropriate Institutional Animal Care and Use Committee (IACUC) following the guidelines of the Animal Welfare Act (AWA)