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Cells were lysed with RIPA assay buffer with added protease (1:100 dilution

52 Contributions of tissue-resident eosinophils to tissue homeostasis Despite the long-observed phenomenon of eosinophil presence in healthy tissues at steady-state conditions, the dogmatic view of eosinophil function is that they evolved exclusively as part of an early innate immune response against parasitic helminths and additionally function in driving pathophysiological responses in type-II disorders such as asthma and EoE where eosinophilia is a common hallmark phenotype

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[DOI] [PubMed] [Google Scholar] 168.Smith JL, Brooker S