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Direct sample application Reliable purification of histidine-tagged proteins directly from unclarified lysatessimply sonicate and run Reduced sample preparation time, which minimizes risk of protein degradation by proteases Simple operation with a syringe, pump, or chromatography system such as KTAdesign or FPLC System Permit rapid yet reliable separations with a minimum of sample preparation and equipment High binding capacity binds up to 40 mg histag protein/mL resin Excellent flow properties great performance even at high flow rates Optimized for unclarified samples short purification time can minimize potential negative effects, such as degradation and oxidation of proteins HiTrap column format compatible with syringe, pump, or chromatography system such as KTA systems or other FPLC systems HisTrap FF crude is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow
MitoTracker Red and immunofluorescence (IF) staining After treatment, the cells were stained with the MitoTracker Red kit (M7512, Invitrogen, Thermo Fisher) in prewarmed HBSS/Ca/Mg for 30 min at 37 C and washed with warm HBSS/Ca/Mg three times
discussion 133-125
